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Short Tandem Repeat Analysis

stranalysis

STR Profile Analysis Services for Cell Line Research and Regenerative Medicine

Short Tandem Repeat (STR) Analysis is used for the identification and authentication of human cell lines in the biotech industry. In eukaryotic genomes, there are repeated polymorphic sequences 2-7 base pairs long and are variable in their number of repeats. Because of this variability, a common core set of STR loci can be utilized for STR analysis in the identification and authentication of cell lines and individuals. Cell line identification and authentication is important to reduce the possibility of using misidentified or contaminated cell lines in research which leads to drastic consequences such as loss of money, time, effort, and integrity of scientific research.

Cell line authentication is the verification of a cell line by matching its profile against the original donor or achieved cell stock. STR analysis is performed when a new cell line is acquired, either from a stock or created in-house, and to verify a cell line’s uniqueness and identity.

This includes:

  • Confirmation of human origin
  • Checking the possibility of contaminating DNA
  • Stability with related cell lines
  • Comparison with a database
  • Verification the cell line is not listed in the ICLAC Register of Misidentified Cell Lines

STR Analysis should be performed when:

  • Working with a new cell stock (created in-house or retrieved from a repository)
  • Beginning a new project
  • Receiving unusual results in experimentation
  • Regular passaging of cell cultures
  • Preparation for grant submission or publishing (NIH Notice)

Cell Line Genetics is a core facility that follows ANSI/ATCC ASN-0002-2021 guidelines. We offer two levels of STR Profile Analysis- STR100 (basic) and STR300 (comprehensive) analysis. Both are designed to accommodate all stages of cell line research including cell line derivation, propagation, and long-term culture. Both STR services include STR profile storage in the Cell Line Genetics’ secure, encrypted database for future profile comparison and 24-hour access to reports on our secure cloud database at no additional charge. STR profiles are generated using Promega’s GenePrint® 24 system which detects all 13 autosomal STR loci recommended for identification and authentication by the updated ANSI/ATCC ASCN-0002 2021 guidelines. The additional 11 loci in the GenePrint® 24 system increases confidence in cell line discrimination, and can resolve null Y allele results for Amelogenin.

STR Profiling 100

Basic service for conducting database cross-referencing, profile comparisons and cross-contamination analysis in-house. Learn More

STR Profiling 300

Comprehensive service designed to authenticate and establish the identity of new cell lines—includes Cellosaurus and Cell Line Genetics database cross-reference analysis.Learn More

 

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