Quality Assurance Package 2
G-Banded Karyotyping |
When to use it...
|
As a baseline screen:
- when cell lines are derived
- before the start of an experiment
- at the conclusion of an experiment
- before publication
- as part of routine cell line QC
- as part of cell banking QC
When cultures show unusual growth properties |
What it detects...
|
Microscopic aberrations >5Mb
Chromosomes abnormalities:
- aneuploidy
- inversions
- duplications/deletions
- translocations
|
What it doesn't detect...
|
Submicroscopic <5Mb in size |
Sample requirements...
|
Live cell culture (T-25 flask)
Whole blood |
Species...
|
Human
Mouse
Rhesus
Rat
Cow
Pig
Horse
Rabbit
Contact us about other species |
Turnaround time...
|
7 - 10 business days
RUSH services available |
Cell Line Identity Testing (STR analysis) |
When to use it...
|
To authenticate cell lines
To establish the identity of newly derived or reprogrammed cell lines
To confirm the relationship of iPS cells to their parental line
To rule out culture cross-contamination |
What it detects...
|
Short tandem repeat polymorphisms (STRs) at 15 loci and Amelogenin
Identify matches between a cell line and an existing STR profile for cell line authentication purposes
Presence of more than one human cell line |
What it doesn't detect...
|
Cell line cross contamination involving other species
Cross contamination of cell lines derived from the same parental line |
Sample requirements...
|
Live cell culture (T-25 flask)
DNA
Frozen Cells |
Species...
|
Human |
Turnaround time...
|
7 - 10 business days
RUSH services available |
Mycoplasma Detection |
When to use it...
|
Routine culture quality control
Rule out mycoplasma contamination when culture characteristics change |
What it detects...
|
19 species of Mycoplasma and 1 species of Acholeplasma (6 of which are responsible for 90-95% of all mycoplasma infections) |
What it doesn't detect...
|
Other less common species |
Sample requirements...
|
T-25 flask or 1.5mL microcentrifuge tube of spent media passaged twice without antibiotics |
Species...
|
All |
Turnaround time...
|
7 - 10 business days |
SKYLite |
When to use it...
|
As an adjunct to G-banding:
. to identify marker chromosomes
. to identify complex chromosome aberrations |
What it detects...
|
Balanced and unbalanced translocations
Aneuploidies |
What it doesn't detect...
|
Submicroscopic genomic abnormalities Inversions , duplications , small deletions |
Sample requirements...
|
Live cell culture (T-25 flask)
Archived cell pellet |
Species...
|
Human |
Turnaround time...
|
7 - 10 business days
RUSH services available |
Custom FISH Probe Construction and Hybridization |
When to use it...
|
To detect chromosome abnormalities - To refine chromosome breakpoints - To screen for aneuploidies and partial trisomies/deletions - To detect emerging abnormal clones |
What it detects...
|
Specific genomic sequence of interest |
What it doesn't detect...
|
Changes in reqions other than the probe-specific region |
Sample requirements...
|
Contact lab |
Species...
|
Most |
Turnaround time...
|
Contact lab |
Transgene Insertation Site Mapping |
When to use it...
|
To confirm integration of transgenes - To determine the number of insertion sites - To localize integration sites to specific chromosomes - To detect interruption of key genes |
What it detects...
|
Integrated sequences >20KB depending on the sequence |
What it doesn't detect...
|
Partial sequence integration - Transgenes or integrations <20KB |
Sample requirements...
|
Contact lab |
Species...
|
Most |
Turnaround time...
|
2 - 4 weeks |
FISH Validation of aCGH Findings |
When to use it...
|
To confirm findings and localize genomic gains detected by a CGH analysis |
What it detects...
|
Chromosome location of genomic gains detected by aCGH - Chromosome location of genomic loss detected by aCGH |
What it doesn't detect...
|
Tandem duplications |
Sample requirements...
|
Contact lab |
Species...
|
Most |
Turnaround time...
|
2 - 4 weeks |
Test |
When to use it...
|
test |
What it detects...
|
test |
What it doesn't detect...
|
test |
Sample requirements...
|
test |
Species...
|
test |
Turnaround time...
|
test |