Quality Assurance Package 1
G-Banded Karyotyping |
When to use it...
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As a baseline screen:
- when cell lines are derived
- before the start of an experiment
- at the conclusion of an experiment
- before publication
- as part of routine cell line QC
- as part of cell banking QC
When cultures show unusual growth properties |
What it detects...
|
Microscopic aberrations >5Mb Chromosomes abnormalities:
- aneuploidy
- inversions
- duplications/deletions
- translocations
|
What it doesn't detect...
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Submicroscopic aberrations |
Sample requirements...
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Live cell culture (T-25 flask) Whole blood |
Species...
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Human
Mouse
Rhesus
Rat
Cow
Pig
Horse
Rabbit
Cat
Contact us about other species |
Turnaround time...
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7 - 10 business days RUSH services available |
Cell Line Identity Testing (STR analysis) |
When to use it...
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To authenticate cell lines To establish the identity of newly derived or reprogrammed cell lines To confirm the relationship of iPS cells to their parental line To rule out culture cross-contamination |
What it detects...
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Short tandem repeat polymorphisms (STRs) at 15 loci and Amelogenin Identify matches between a cell line and an existing STR profile for cell line authentication purposes Presence of more than one human cell line |
What it doesn't detect...
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Cell line cross contamination involving other species Cross contamination of cell lines derived from the same parental line |
Sample requirements...
|
Live cell culture (T-25 flask) DNA Frozen Cells |
Species...
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Human |
Turnaround time...
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7 - 10 business days RUSH services available |
Standard FISH (fluorescence in situ hybridization) |
When to use it...
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As an adjunct to G-banding :
- to identify complex chromosome aberrations
- to identify small partial trisomies
- to identify small marker chromosomes
- to refine chromosome breakpoints
|
What it detects...
|
Genomic sequences of interest:
- duplications and deletions >40KB
- low level mosaicism
- unbalanced translocations
- cryptic chromosome aberrations
|
What it doesn't detect...
|
Chromosome aberrations other than the probe sequence of interest |
Sample requirements...
|
Live cell culture (T-25 flask) Archived cell pellet |
Species...
|
Human Mouse Contact us about other species |
Turnaround time...
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7-10 business days RUSH services available |
QuickFISH |
When to use it...
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As a rapid trisomy screen for large numbers of cultures adjunct to G-banding As an adjunct to routine G-banding to detect small emerging populations of trisomic cells |
What it detects...
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Most common trisomies found in human IPS and ESC lines (Trisomy 12 and 17) Most common trisomies found in mouse IPS and ESC lines (Trisomy 8 and 11) |
What it doesn't detect...
|
Structural chromosome aberrations Other trisomies or partial trisomies |
Sample requirements...
|
Live cell culture (T-25 flask) Frozen cells |
Species...
|
Human Mouse |
Turnaround time...
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2 - 3 business days RUSH services available |
Mycoplasma Detection |
When to use it...
|
Routine culture quality control Rule out mycoplasma contamination when culture characteristics change |
What it detects...
|
11 species of Mycoplasma and 1 species of Ureaplasma . most common Mycoplasma contaminants in cell cultures |
What it doesn't detect...
|
Other less common species |
Sample requirements...
|
T-25 flask grown 2 passages without antibiotics |
Species...
|
All |
Turnaround time...
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7 - 10 business days |
SKYLite |
When to use it...
|
As an adjunct to G-banding: . to identify marker chromosomes . to identify complex chromosome aberrations |
What it detects...
|
Balanced and unbalanced translocations Aneuploidies |
What it doesn't detect...
|
Submicroscopic genomic abnormalities |
Sample requirements...
|
Live cell culture (T-25 flask) Archived cell pellet |
Species...
|
Human |
Turnaround time...
|
7 - 10 business days RUSH services available |
Custom FISH Probe Construction and Hybridization |
When to use it...
|
To detect chromosome abnormalities - To refine chromosome breakpoints - To screen for aneuploidies and partial trisomies/deletions - To detect emerging abnormal clones |
What it detects...
|
Specific genomic sequence of interest |
What it doesn't detect...
|
Changes in reqions other than the probe-specific region |
Sample requirements...
|
Contact lab |
Species...
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Most |
Turnaround time...
|
Contact lab |
Transgene Insertation Site Mapping |
When to use it...
|
To confirm integration of transgenes - To determine the number of insertion sites - To localize integration sites to specific chromosomes - To detect interruption of key genes |
What it detects...
|
Integrated sequences >20KB depending on the sequence |
What it doesn't detect...
|
Partial sequence integration - Transgenes or integrations <20KB |
Sample requirements...
|
Contact lab |
Species...
|
Most |
Turnaround time...
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2 - 4 weeks |
FISH Validation of aCGH Findings |
When to use it...
|
To confirm findings and localize genomic gains detected by a CGH analysis |
What it detects...
|
Chromosome location of genomic gains detected by aCGH - Chromosome location of genomic loss detected by aCGH |
What it doesn't detect...
|
Tandem duplications |
Sample requirements...
|
Contact lab |
Species...
|
Most |
Turnaround time...
|
2 - 4 weeks |
Test |
When to use it...
|
test |
What it detects...
|
test |
What it doesn't detect...
|
test |
Sample requirements...
|
test |
Species...
|
test |
Turnaround time...
|
test |