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Services > Full Cell Line Characterization/Authentication Package 1
Full Cell Line Characterization/Authentication Package 1 includes the following services:
| G-Banded Karyotyping
|
When to use it... |
As a baseline screen:
· when cell lines are derived
· before the start of an experiment
· at the conclusion of an experiment
· before publication
· as part of routine cell line QC
· as part of cell banking QC
When cultures show unusual growth properties |
What it detects... |
Microscopic aberrations >5Mb
Chromosomes abnormalities:
. aneuploidy
· inversions
· duplications/deletions
· translocations
<5% mosaicism for specific trisomies
<10% mosaicism for other aberrations |
What it doesn't detect... |
Submicroscopic aberrations <5Mb in size |
Sample requirements... |
Live cell culture (T-25 flask)
Whole blood |
Species... |
Human
Mouse
Rhesus
Rat
Cow
Pig
Horse
Rabbit
Contact us about other species |
Turnaround time... |
7 - 10 business days
RUSH services available |
| Cell Line Identity Testing (STR analysis)
|
When to use it... |
To authenticate cell lines
To establish the identity of newly derived or reprogrammed cell lines
To confirm the relationship of iPS cells to their parental line
To rule out culture cross-contamination |
What it detects... |
Short tandem repeat polymorphisms (STRs) for 15 loci plus Amelogenin
Identify match between a cell line and an existing STR profile for cell line authentication purposes
Presence of more than one cell line of the same species |
What it doesn't detect... |
Cell line cross contamination involving non-human species
Cross contamination of cell lines derived from the same parental line |
Sample requirements... |
Live cell culture (T-25 flask)
DNA
Frozen Cells |
Species... |
Human |
Turnaround time... |
7 - 10 business days
RUSH services available |
| Standard FISH (fluorescence in situ hybridization)
|
When to use it... |
As an adjunct to G-banding :
. to identify complex chromosome aberrations
. to identify small partial trisomies
. to identify small marker chromosomes
. to refine chromosome breakpoints |
What it detects... |
Genomic sequences of interest:
. duplications and deletions >20KB
. mosaicism of <2% (e.g., 4 of 200 interphase cells with trisomy 12)
. unbalanced translocations
. cryptic chromosome aberrations |
What it doesn't detect... |
Chromosome aberrations other than the probe sequence of interest |
Sample requirements... |
Live cell culture (T-25 flask)
Archived cell pellet |
Species... |
Human |
Turnaround time... |
5 - 10 business days
RUSH services available |
| QuickFISH
|
When to use it... |
As a rapid trisomy screen for large numbers of cultures adjunct to G-banding
As an adjunct to routine G-banding to detect small emerging populations of trisomic cells |
What it detects... |
Most common trisomies found in human IPS and ESC lines (Trisomy 12 and 17)
Most common trisomies found in mouse IPS and ESC lines (Trisomy 8 and 11) |
What it doesn't detect... |
Structural chromosome aberrations
Other trisomies or partial trisomies |
Sample requirements... |
Live cell culture (T-25 flask)
Frozen cells |
Species... |
Human
Mouse |
Turnaround time... |
2 - 3 business days
RUSH services available |
| Mycoplasma Detection
|
When to use it... |
Routine culture quality control
Rule out mycoplasma contamination when culture characteristics change |
What it detects... |
11 species of Mycoplasma and 1 species of Ureaplasma
. most common Mycoplasma contaminants in cell cultures |
What it doesn't detect... |
Other less common species |
Sample requirements... |
T-25 flask grown 2 passages without antibiotics |
Species... |
All |
Turnaround time... |
7 - 10 business days |
| SKYLite
|
When to use it... |
As an adjunct to G-banding:
. to identify marker chromosomes
. to identify complex chromosome aberrations |
What it detects... |
Balanced and unbalanced translocations
Aneuploidies |
What it doesn't detect... |
Submicroscopic genomic abnormalities <5KB
Inversions , duplications , small deletions |
Sample requirements... |
Live cell culture (T-25 flask)
Archived cell pellet |
Species... |
Human |
Turnaround time... |
7 - 10 business days
RUSH services available |
| Custom FISH Probe Construction and Hybridization
|
When to use it... |
To detect chromosome abnormalities - To refine chromosome breakpoints - To screen for aneuploidies and partial trisomies/deletions - To detect emerging abnormal clones |
What it detects... |
Specific genomic sequence of interest |
What it doesn't detect... |
Changes in reqions other than the probe-specific region |
Sample requirements... |
Contact lab |
Species... |
Most |
Turnaround time... |
Contact lab |
| Transgene Insertation Site Mapping
|
When to use it... |
To confirm integration of transgenes - To determine the number of insertion sites - To localize integration sites to specific chromosomes - To detect interruption of key genes |
What it detects... |
Integrated sequences >20KB depending on the sequence |
What it doesn't detect... |
Partial sequence integration - Transgenes or integrations <20KB |
Sample requirements... |
Contact lab |
Species... |
Most |
Turnaround time... |
2 - 4 weeks |
| FISH Validation of aCGH Findings
|
When to use it... |
To confirm findings and localize genomic gains detected by a CGH analysis |
What it detects... |
Chromosome location of genomic gains detected by aCGH - Chromosome location of genomic loss detected by aCGH |
What it doesn't detect... |
Tandem duplications |
Sample requirements... |
Contact lab |
Species... |
Most |
Turnaround time... |
2 - 4 weeks |
| Test
|
When to use it... |
test |
What it detects... |
test |
What it doesn't detect... |
test |
Sample requirements... |
test |
Species... |
test |
Turnaround time... |
test |
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