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Quality Assurance Package 2

G-Banded Karyotyping

When to use it...

As a baseline screen:

  • when cell lines are derived
  • before the start of an experiment
  • at the conclusion of an experiment
  • before publication
  • as part of routine cell line QC
  • as part of cell banking QC

When cultures show unusual growth properties

What it detects...

Microscopic aberrations >5Mb

Chromosomes abnormalities:

  • aneuploidy
  • inversions
  • duplications/deletions
  • translocations

What it doesn't detect...

Submicroscopic <5Mb in size

Sample requirements...

Live cell culture (T-25 flask)

Whole blood

Species...

Human Mouse Rhesus Rat Cow Pig Horse Rabbit Contact us about other species

Turnaround time...

7 - 10 business days RUSH services available

Cell Line Identity Testing (STR analysis)

When to use it...

To authenticate cell lines To establish the identity of newly derived or reprogrammed cell lines To confirm the relationship of iPS cells to their parental line To rule out culture cross-contamination

What it detects...

Short tandem repeat polymorphisms (STRs) at 15 loci and Amelogenin Identify matches between a cell line and an existing STR profile for cell line authentication purposes Presence of more than one human cell line

What it doesn't detect...

Cell line cross contamination involving other species Cross contamination of cell lines derived from the same parental line

Sample requirements...

Live cell culture (T-25 flask) DNA Frozen Cells

Species...

Human

Turnaround time...

7 - 10 business days RUSH services available

Mycoplasma Detection

When to use it...

Routine culture quality control Rule out mycoplasma contamination when culture characteristics change

What it detects...

19 species of Mycoplasma and 1 species of Acholeplasma (6 of which are responsible for 90-95% of all mycoplasma infections)

What it doesn't detect...

Other less common species

Sample requirements...

T-25 flask or 1.5mL microcentrifuge tube of spent media passaged twice without antibiotics

Species...

All

Turnaround time...

7 - 10 business days


Read More: Printable Services Overview

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