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Quality Assurance Package 1

G-Banded Karyotyping

When to use it...

As a baseline screen:

  • when cell lines are derived
  • before the start of an experiment
  • at the conclusion of an experiment
  • before publication
  • as part of routine cell line QC
  • as part of cell banking QC

When cultures show unusual growth properties

What it detects...

Microscopic aberrations >5Mb Chromosomes abnormalities:

  • aneuploidy
  • inversions
  • duplications/deletions
  • translocations

What it doesn't detect...

Submicroscopic aberrations

Sample requirements...

Live cell culture (T-25 flask) Whole blood

Species...

Human Mouse Rhesus Rat Cow Pig Horse Rabbit Cat Contact us about other species

Turnaround time...

7 - 10 business days RUSH services available

Cell Line Identity Testing (STR analysis)

When to use it...

To authenticate cell lines To establish the identity of newly derived or reprogrammed cell lines To confirm the relationship of iPS cells to their parental line To rule out culture cross-contamination

What it detects...

Short tandem repeat polymorphisms (STRs) at 15 loci and Amelogenin Identify matches between a cell line and an existing STR profile for cell line authentication purposes Presence of more than one human cell line

What it doesn't detect...

Cell line cross contamination involving other species Cross contamination of cell lines derived from the same parental line

Sample requirements...

Live cell culture (T-25 flask) DNA Frozen Cells

Species...

Human

Turnaround time...

7 - 10 business days RUSH services available

Standard FISH (fluorescence in situ hybridization)

When to use it...

As an adjunct to G-banding :

  • to identify complex chromosome aberrations
  • to identify small partial trisomies
  • to identify small marker chromosomes
  • to refine chromosome breakpoints

What it detects...

Genomic sequences of interest:

  • duplications and deletions >40KB
  • low level mosaicism
  • unbalanced translocations
  • cryptic chromosome aberrations

What it doesn't detect...

Chromosome aberrations other than the probe sequence of interest

Sample requirements...

Live cell culture (T-25 flask) Archived cell pellet

Species...

Human Mouse Contact us about other species

Turnaround time...

7-10 business days RUSH services available


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