StemArray

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Why StemArray™ is right for you...

StemArray™: full genome, high resolution stem cell focused comparative genomic hybridization array with increased probe coverage in over 60 stem cell associated genes and more than 200 cancer related genes.


Custom-designed for human iPS and ES cells, CLG's StemArray™ offers a reproducible, high-resolution karyotyping solution for the detection of unbalanced structural and numerical chromosomal alterations with high-throughput capabilities.

• 12x135K Roche Nimblegen aCGH platform

• Increased probe coverage in over 60 pluripotent associated genes

• Increased probe coverage in over 200 cancer associated genes

• High resolution full genome coverage with 15 KB overall median probe spacing for detection of unbalanced structural and numerical chromosomal alterations

• Detectable mosaicism level at 25 to 35%

• Highly Reproducible, fast turnaround time, no cell culture needed

• Minimum of 500ng of DNA required

Previous research has shown that human pluripotent stem cells in long-term culture acquire chromosomal changes similar to those seen during tumorigenesis [1,2,]. Therefore, it is essential that an array used for characterizing human embryonic and induced pluripotent stem cells has the ability to detect copy number changes in stemness and cancer related genes as these aberrations will undoubtedly impact the survival and proliferative abilities of the cells.



References:

1. Baker, D. E. C., Harrison, N. J., Maltby, E., Smith, K., Moore, H. D., Shaw, P. J., et al. (2007). Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Nature Biotechnology, 25, 207–215.
2.Yang, S., Lin, G., Tan, Y., Zhou, Di., Deng, L., Cheng, D., et al. (2008). Tumor progression of culture-adapted human embryonic stem cells during long-term culture. Genes Chromosomes Cancer, 47, 665–679.
Previous research has shown that human pluripotent stem cells in long-term culture acquire chromosomal changes similar to those seen during tumorigenesis [1,2,]. Therefore, it is essential that an array used for characterizing human embryonic and induced pluripotent stem cells has the ability to detect copy number changes in stemness and cancer related genes as these aberrations will undoubtedly impact the survival and proliferative abilities of the cells.



References:

1. Baker, D. E. C., Harrison, N. J., Maltby, E., Smith, K., Moore, H. D., Shaw, P. J., et al. (2007). Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Nature Biotechnology, 25, 207–215.
2.Yang, S., Lin, G., Tan, Y., Zhou, Di., Deng, L., Cheng, D., et al. (2008). Tumor progression of culture-adapted human embryonic stem cells during long-term culture. Genes Chromosomes Cancer, 47, 665–679.

StemArray™ is available for direct sale or as a Cell Line Genetics quality assurance service

 

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"Our lab has relied on Cell Line Genetics for stem cell characterization, authentication and custom services for over 3 years. CLG has consistently provided the level of service, expertise and turnaround our research efforts demand.

Cell Line's characterization and detection of polymorphisms of our non-human primate induced pluripotent stem cell lines (iPSC) was an important part of our research effort.

We value our relationship with CLG and look forward to our continued partnership."


- Fred H. Gage, Ph.D.
The Salk Institute for Biological Studies