Multispecies G-band Karyotyping Analysis
Cell Line Genetics is recognized world-wide for its expertise in multispecies stem cell and cancer cell line karyotyping analysis. Unlike most cytogenetic core facilities focused on clinical testing, Cell Line Genetics is dedicated to providing the stem cell research and regenerative medicine field the highest quality g-band karyotyping service available with fast turn-around garneted. Furthermore, Cell Line Genetics scientists are rigorously trained and experienced at stem cell genomic stability analysis and identifying structural chromosomal anomalies unique to multipotent and pluripotent stem cell lines—many of which can lead to invasive cell populations. Since the company’s inception, Cell Line Genetics has characterized thousands of adult, embryonic and induced pluripotent stem cells samples from leading academic institutions, government agencies and industry stem cell labs. Request an estimate today and have Cell Line Genetics become your g-band karyotyping quality assurance partner.
• Harvesting of live cell cultures for chromosome analysis
• Live cell cultures and metaphase cell pellets accepted
• Chromosome analysis of 20 cells per cell line
• High quality g-band karyotypes in publication ready format
• Comprehensive interpretation of results by CLG scientist
• Report signed by clinically certified cytogeneticist
• Results securely stored in CLG database
• 7-10 day turn around—rush services are available
• Service complies with FDA Good Laboratory Practice (GLP)
Used to: |
As a baseline screen: |
Detects: |
Microscopic aberrations >5Mb |
Doesn’t Detect: |
Submicroscopic aberrations <5Mb in size |
Species: |
Human |
Turnaround: |
7 - 10 business days |
PROCEDURE: Mailing Live Cultures for Karyotyping [Print Sample Requirements]
Requisition Form
Each sample must be accompanied by its own Test Requisition Form. The test requisition form can be downloaded here.
Preparing Culture for Mailing
1. Passage the cells to a T-25 culture flask as you would under normal conditions. Seed the cells dense enough so that under the microscope the culture appears “peppered” with cells cell clusters. Please Note: If you are growing cells on a feeder layer, make sure the feeder layer is fresh.
2. Feed the culture as usual. The culture is ready to send when the colonies are readily visible by eye and the culture appears “peppered” with “pinpoint” size colonies.
3. The cultures must be in log phase when you mail them.
Mailing Instructions:
1. Fill the culture flask to the top with complete media, tighten the cap, and seal with parafilm.
2. Place 25 ml complete media in a T-25 flask or 50 mL centrifuge tube, and seal with parafilm.
3. Place both the flask and the extra media in a sealed plastic bag and wrap the bag in bubble wrap.
4. Complete a Test Requisition for EACH cell line you are sending for analysis.
5. Place the Test Requisition, culture flask, and 25 ml of complete media in a padded mailing container or box.
6. Send the mailer to Cell Line Genetics by FedEx® or other next-day delivery service.
7. SHIP AT ROOM TEMPERATURE. Do not ship on ice packs or cold packs .
8. Call our laboratory when you are ready to mail the package.
Please Note: CLG does not accept specimens delivered on Saturday
| Mailing Address | Contact Information |
| Attention Julie Johnson Cell Line Genetics 510 Charmany Drive, Suite 254 Madison, WI 53719 | Julie Johnson Director of Laboratory Operations Lab Phone: (608) 441-8163 FAX: (608) 441-8162 Email: jjohnson@clgenetics.com |
Request an Estimate
Our Experience:
Based on testing over 7500 stem cell samples, chromosomal abnormalities were detected in:
-70% of mouse iPSC lines
-61% of mouse ESC lines
-28% of human ESC lines
-22% of human iPSC lines
From Our Customers
"CLG has served our karyotyping and fluorescence in situ hybridization needs from early discovery and product development through clinical trials.
We depend on CLG’s rapid delivery of results and state of the art analyses to ensure that our products are the highest caliber possible. CLG has been our number one choice in cell authentication and cytogenetics for over 5 years."
- Lynn Allen-Hoffmann, Ph.D.
CEO, Stratatech Corporation